The Combination Effect of Collagen-Chitosan Hydrogel and Injectable Platelet-Rich Fibrin Fractionation on Osteoblast Migration and Proliferation



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Submitted Mar 23, 2023
Published Jun 14, 2023
10.24018/ejdent.2023.4.3.264

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Injectable Platelet Rich Fibrin (i-PRF) is a popular periodontal regenerative therapy in dentistry. i-PRF centrifugation resulted in the fractionation of the yellow layer and buffy coat with a red layer. The red i-PRF contains a greater number of Growth Factor carrier cells than yellow layer, besides that liquid-PRF has a suitable consistency so it easy to combine with other biomaterials. Another biomaterial that can be combined with i-PRF is collagen-chitosan with hydrogel preparations. This study aims to determine the effect of a combination of collagen-chitosan hydrogel formulation with injection of platelet-rich fibrin fractionation on osteoblast cell migration and proliferation. This type of research was an experimental laboratory divided into 3 groups, which were given a combination of the collagen-chitosan hydrogel and i-PRF red layer, a combination of collagen-chitosan hydrogel and i-PRF yellow layer, and only a combination of collagen-chitosan hydrogel. Chitosan collagen hydrogel was prepared with a collagen/chitosan ratio of 25/75, then mixed with i-PRF fractionation with a ratio of 1:1. The cell proliferation test used CCK-8, while the cell migration test used scratch wound healing. Data were tested with One way ANOVA for cell migration and Two way ANOVA for cell proliferation, followed by a Post Hoc test. The results showed that migration of osteoblast cells after 24 hours in the combination group of chitosan collagen hydrogel and i-PRF red layer was higher than the other groups with significance (p<0.05). The results of osteoblast cell proliferation on days 1, 3, and 5 in the combination group of chitosan collagen hydrogel and i-PRF red layer were also higher than the other groups with significance (p<0.05). This study concluded that the combination of chitosan collagen hydrogel with injectable platelet-rich fibrin red layer had a more effect on increasing the migration and proliferation of osteoblast cells than the other groups.

Keywords: Collagen Chitosan hydrogel injectable platelet-rich fibrin

References

  1. Mohan SP, Jaishangar N, Devy S, Narayanan A, Cherian D, Madhavan SS. Platelet-Rich Plasma and Platelet-Rich Fibrin in Periodontal Regeneration: A Review. J Pharm Bioallied Sci. 2019; 11(2); 126-130.
     Google Scholar
  2. Wang X, Zhang Y, Choukroun J, Ghanaati S, Miron RJ. Effects of injectable platelet-rich fibrin on osteoblast behaviour and bone tissue formation in comparison to platelet-rich plasma. Platelets: Early Online. 2017; 1-8.
     Google Scholar
  3. Hassan H, Quinlan DJ, Ghanem A. Injectable Platelet-Rich Fibrin for Facial Rejuvenation: A Prospective, Single-Center Study. J Cosmet Dermatol. 2020; 19(2); 3213-3221.
     Google Scholar
  4. Thanasrisuebwong P, Kiattavorncharoen S, Surarit R, Phruksaniyom C, Ruangsawasdi N. Red and Yellow Injectable Platelet-Rich Fibrin Demonstrated Differential Effects on Periodontal Ligament Stem Cell Proliferation, Migration, and Osteogenic Differentiation. Int J Mol Sci. 2020; 21(14): 5153.
     Google Scholar
  5. Olivia N, Kusumadewi WW, Sumito N, Murdiastuti K. Osteoblast Migration Effect of the Freeze-Dried Homologous Platelet Rich Plasma. Indian Journal of Public Health Research & Development. 2021; 12(3): 446-452.
     Google Scholar
  6. Varela HA, Souza JCM, Nascimento RM, Araaojo JrRF, Vasconcelos RC, Cavalcante RS, et al. Injectable platelet-rich fibrin: cell content, morphological, and protein characterization, Clin Oral Invest. 2018.
     Google Scholar
  7. Mu Z, Chen K, Yuan S, Li Y, Huang Y, Wang C, et al. Gelatin Nanoparticle Injectable Platelet Rich Fibrin Double Network Hydrogels with Local Adaptability and Bioactivity for Enhanced Osteogenesis. Adv. Healthcare Mater. 2020; 9(5); 1901469.
     Google Scholar
  8. Anindyajati TB, Lastianny SP, Yogianti F, Murdiastuti K. Effect of collagen-chitosan hydrogel formula combined with platelet-rich plasma (A study of pH, viscocity, and swelling test). Maj Ked Gi Ind. 2020; 6(3): 123-128.
     Google Scholar
  9. Osidak E, Osidak M, Akhmanova M, Domogatskii S. Collagen A Biomaterial for Delivery Of Growth Factor and Tissue Regeneration. Russian Journal of General Chemistry. 2014; 84(2); 368-378.
     Google Scholar
  10. Rosdiani, AF, Widiyanti P, Rudyarjo DI. Synthesis and characterization biocomposite collagen-chitosan-glycerol as scaffold for gingival recession therapy. Journal of International Dental and Medical Research. 2017; 10(1); 118-122.
     Google Scholar
  11. Deng C, Zhang P, Vulesevic B, Kuraitis D, Li F, Yang AF, et al. A Collagen-Chitosan Hydrogel for Endothelial Differentiation and Angiogenesis. Tissue Eng A Part. 2010; 16(10); 3099-109.
     Google Scholar
  12. Czekanska EM, Stoddart MJ, Richards RG, Hayes JS. In Search Of An Osteoblast Cell Model For In Vitro Research. ECM Journal. 2012; 24: 1-17.
     Google Scholar
  13. Hui E, Lerouge S. Mechanical and biological properties of chitosan/purecol collagen hidrogels. J of Rheology. 2017;30(2): 1-9.
     Google Scholar
  14. Man K, Brunet MY, Federici AS, Hoey DA, Cox SC. An ECM-Mimetic Hydrogel to Promote the Therapeutic Efficacy of Osteoblast-Derived Extracellular Vesicles for Bone Regeneration. Front. Bioeng. Biotechnol. 2022; 10: 829969
     Google Scholar
  15. Dandastri AA, Suryono, Murdiastuti K. The Influence Between Injectable Platelet-Rich Fibrin and Platelet-Rich Plasma Towards Gingival Fibroblast Cell Proliferation. Odonto Dental Journal. 2021; 8(2): 25-31.
     Google Scholar
  16. Farshidfar N, Jafarpour D, Firoozi P, Sahmeddini S, Hameandi S, Tayebi L, et al. The application of injectable platelet-rich fibrin in regenerative dentistry: A systematic scoping review of In vitro and In vivo studies. Japanese Dental Science Review. 2022; 58; 89-123.
     Google Scholar
  17. Olivia N, Kusumadewi WW, Sumito N, dan Murdiastuti K. Osteoblast Migration Effect of the Freeze-Dried Homologous Platelet Rich Plasma. Indian Journal of Public Health Research & Development. 2021; 12(3): 446-452.
     Google Scholar
  18. Man K, Brunet MY, Federici AS, Hoey DA, Cox SC. An ECM-Mimetic Hydrogel to Promote the Therapeutic Efficacy of Osteoblast-Derived Extracellular Vesicles for Bone Regeneration. Front. Bioeng. Biotechnol. 2022; 10: 829969.
     Google Scholar
  19. Dallas SL, Bonewald LF. Dynamics of the transition from osteoblast to osteocyte. Annals of the New York Academy of Sciences. 2010; 1192(1): 437-443.
     Google Scholar
  20. Danastri AA, Suryono MK. The Influence Between Injectable Platelet-Rich Fibrin and Platelet-Rich Plasma Towards Gingival Fibroblast Cell Proliferation. Odonto Dental Journal. 2021; 8(2): 25-31.
     Google Scholar
  21. Farshidfar N, Jafarpour D, Firoozi P, Sahmeddini S, Hamedani S, Freitas de Souza R, et al. The application of injectable platelet-rich fibrin in regenerative dentistry: A systematic scoping review of In vitro and In vivo studies. Japanese Dental Science Review. 2022; 58: 89-123.
     Google Scholar